The Logical Reasoning in Human Genetics course lecture 17 took place on March 1st ⏰9 AM (ET) | 3 PM (GMT+1).
The title of the lecture was: ‘Exploring the oral microbiota by metagenomics appraoch: from Bacteria to Archaea’ and it was delivered by Dr. Feriel Bouzid, post-doctoral researcher at the Laboratory of Molecular and Cellular Screening Processes (LPCMC) at the centre of biotechnology of Sfax, Tunisia, and moderated by Ahmed Rebai, Professor at Centre of Biotechnology of Sfax.
Watch the full recording here.
The oral cavity contains one of the most diverse and unique microbial communities in the human body. The dynamic and polymicrobial oral microbiome can shift from homeostasis to dysbiosis due to infections and other stress factors. The oral microbiome dysbiosis can predispose to other systemic diseases in distal sites like cardiovascular diseases. We assessed the oral microbiota composition variation between Tunisian coronary artery disease (CAD) patients and healthy subjects to identify a potential pathogenic signature associated with CAD. Targeted metagenomics approach was adopted which involves 16S ribosomal RNA (rRNA) next-generation sequencing. We detected a different abundance distribution of the genus Eikenella in oral samples of coronary patients compare to controls for the first time compared to equivalent studies. We also showed the presence of methanogens in the oral microbiota with an unprecedented relatively high prevalence using a polyphasic molecular approach. We noticed that metagenomic 16S rRNA sequencing still overlook the human archaeome and partially failed to picture the diversity of archaeal signatures. We discuss technical aspects, bioinformatics and biostatistics analyses that have shaped our understanding of the human microbiome. We also identify future challenges to integrate human and microbial genomic data sets to predict risk of human diseases.
About the Lecturer:
Fériel BOUZID has received a PhD degree in biology-microbiology from the University Aix Marseille, France. She is currently a post-doctoral researcher at the Laboratory of Molecular and Cellular Screening Processes (LPCMC) at the centre of biotechnology of Sfax, Tunisia.
Fériel is working on the human microbiome profiling by next-generation sequencing to detect potential microbial signatures, with focus on cardiovascular diseases. Her expertise also includes metagenomic data analysis by bioinformatics pipelines.
Her previous research experience have provided skills on molecular diagnostic tools development for human infectious diseases and pathogen-host interaction studies through animal and cellular models.
The LRHG course is in partnership with Institut Pasteur de Tunis - معهد باستور تونس . You can join us every first Wednesday of the month for a new and enlightening lecture ??
More information on the course and recordings of the previous lectures can be found here.
